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»  Projects

2000 »  Production of pigments with microalgae (AIF ProInno Project).

»  Establishment of a plant in Büsum for the production of microalgae and microalgae specialities (EU FIAF Project).

2001 »  Expression of isotopically tagged proteins in cyanobacteria (Innovationsstiftung Schleswig-Holstein).
2002 »  Conception and realisation of a pilot plant in 2.000,00Liters scale for the upscale of the laboratory process for production of Selenium and Zink Spirulina (EU FIAF Project).

2003 »  Solar based algae production (AIF ProInno Project).

»  Development of a process and operation of a pilot plant for the organic production of spirulina microalgae (EU FIAF Project).

»  ACCELERATED DETOXIFICATION SYSTEM FOR LIVE MARINE SHELLFISH CONTAMINATED BY PSP TOXINS (EU CRAFT (Kooperationsforschung) Projekt).

»  Development of methods for the isolation and analysis of Yessotoxin (Federal Institute for Risk Assessment).

2004 »  Development of methods for the isolation and analysis of Yessotoxin-Derivates (45-OH-YTX, Homo-YTX and 45-OH-Homo-YTX) (Federal Institute for Risk Assessment).

2005 »  ProMiTox: Produktion of microalgae toxins (Company Innovation-Basic Innovation WTSH).

»  Acceleration of hydrogenproduction in Cyanobacteria (HWT-Program, Innovationsstiftung Schleswig-Holstein).

2008 »  An Innovation assistant was founded in our company through the Future program „Economics“ (WTSH).

2010 »  Application of Pavlova microalgae as alternative feed in larval rearing (Deutsche Bundesstiftung Umwelt).
2012 »  Development of Microalgae concentrates as feed for Copepods for application in marine aquaculture (MiCo). (Federal office of Agriculture and Food).

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Title:
Conception and development of an innovative process for the production of microalgae pigments through realisation of suitable cultivation conditions by an innovative bioreactor system developed by FairMenTec for the production of the algae pigments and an extraction method developed by BlueBioTech.

Short title: Pigment extraction from microalgae.

Duration: 01.12.2000 – 31.03.2003.

Description:

Funding:

Im Rahmen von PRO INNO  der Arbeitsgemeinschaft industrieller Forschungsvereinigungen (AiF).

 

Project partners:

FairMenTec GmbH
Berliner Straße 1
37073 Göttingen
www.Fairmentec.de

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Title:
Establishment of a plant in Büsum for the production of microalgae and microalgae specialities (EU FIAF Project).

Duration: 08.12.2000 – 31.12.2003

Description:

The aim of this project was to make use of the scientific know how from basic and applied research on marine biotechnology for the development of new products. Within the project modular and flexible photobioreactors were installed. This enables BlueBioTech to conduct process development, contract research and optimisation of cultivation strategies.

 
Funding:

Tis project was funded by the European Community and by the federal state of Schleswig Holstein under the „Finanzinstruments für die Ausrichtung der Fischerei“ (FIAF) programme.

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Title: Expression of isotopically tagged proteins in cyanobacteria (Innovationsstiftung Schleswig-Holstein)

Duration: 01.10.2001 – 31.09.2004

Description:
The aim of this project was to widen the application of NMR (Nuclear Magnetic Resonance)-Spectroscopy through development of an expression system in the cyanobacterium Synechocystis sp. Within the project BBT developed a cultivation technology for the cost efficient production of isotopically tagged proteins.

Funding: Innovationsstiftung Schleswig-Holstein.

Project partners: Christian-Albrechts-Universität, AG Schulz, Herr Prof. Dr. Rüdiger Schulz.


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Title:
Conception and realisation of a pilot plant in 2.000,00Liters scale for the upscale of the laboratory process for production of Selenium and Zink Spirulina (EU FIAF Project).

Duration: 08.01.2002 – 30.06.2004

Description:
Within this project cultivation technologies for the production of Spirulina microalgae enriched with several micronutrients (Zn, Se, Cr, Fe) were developed and realised. Through specific and highly accurate process control in combination with continuous monitoring of growth conditions the cultivation conditions could be standardised. This ensures reproducible growth conditions and a consistently high product quality. The results of this project will be included in the BluBio product range.

Funding:

Tis project was funded by the European Community and by the federal state of Schleswig Holstein under the „Finanzinstruments für die Ausrichtung der Fischerei“ (FIAF) programme.

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Title:
Identification of Haematococcus pluvialis strains suitable for outdoor cultivation with special attention to outdoor specific conditions including development of processes and product formulations for the extraction and stabilisation of astaxanthin.

Short title: Solar based algae production.

Duration: 01.04.2003 – 30.06.2005.

Description:

Within the project a multi stage cultivation process was developed that ensures reliable production of the microalgae Haematococcus at competitive quality level.

Funding:

by the PRO INNO  der Arbeitsgemeinschaft industrieller Forschungsvereinigungen (AiF).

Project partners:

King Dharmsa Spirulina Company
Fuqing City, Fujian Province
China


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Title: Development of a process and operation of a pilot plant for the organic production of spirulina microalgae.

Descprition:
Within this project a cultivation process for the production of Spirulina according to Naturland guidelines was developed.

Duration: 26.8.2003 – 1.12.2007

Tis project was funded by the European Community and by the federal state of Schleswig Holstein under the „Finanzinstruments für die Ausrichtung der Fischerei“ (FIAF) programme.

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Title: ACCELERATED DETOXIFICATION SYSTEM FOR LIVE MARINE SHELLFISH CONTAMINATED BY PSP TOXINS.

Duration: 01.02.2003-31.01.2006

Description:
Project for the research and development of an accelerated detoxification system for marine shellfish contaminated by PSP algae toxins.

Funding:

Project partner in the EU CRAFT (Co-operative Research Projects) Project

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Title: Development of methods for the isolation and analysis of Yessotoxin.

Duration: 1/2003 – 10/2004.

Description:
The aim of the project „Development of methods for the isolation and analysis of Yessotoxin“ could be realised. Isolated Yessotoxin could be quantified, stored in definite amounts and made available to the analysis of food.

Project partners:

TeLA GmbH Bremerhaven
Fischkai 1
D-27572 Bremerhaven

Funding:
Federal Institute for Risk Assessment.


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Title: Development of methods for the isolation and analysis of Yessotoxin-Derivates (45-OH-YTX, Homo-YTX and 45-OH-Homo-YTX).

Duration: 2004 / 2005.

Description:
Within this project the Yessotoxin derivates 45-OH-YTX, Homo-YTX and 45-OH-Homo-YTX that are covered by decision of the EU-Commission 2002/225/EG from 15.03.02 should be produced, chemically characterised, isolated and purified in addition to Yessotoxin. In conclusion it can be stated that it is possible to produce and purify YTX from specific microalgae in order to provide standard material for analysis. However the production and purification from YTX derivates ( 45-OH-YTX, Homo-YTX and 45-OH-Homo-YTX) is currently not feasable. Despite application of the SIM-method we were not able to detect YTX derivates in the microalgae. Probably these derivates are the result of metabolic processing of YTX in the shellfish.

Project partners:

TeLA GmbH Bremerhaven
Fischkai 1
D-27572 Bremerhaven

Funding:
Federal Institute for Risk Assessment.

.


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Title: ProMiTox: Produktion of microalgae toxins.

Duration: 1.4.2005 – 31.7.2007.

Description:
Algae toxins are substances with highly toxic potential that are produced by several microalgae and can be accumulated in seafood as fish and shellfish. As these toxins present serious health risks, the monitoring of seafood regarding the contamination with microalgae toxins is regulated in the EU and many other countries worldwide. For this monitoring the microalgae toxins are needed in highly purified form as analytical standard material for calibration of the analysis procedures. Compared to the worldwide request the supply of these toxin stands is suboptimal. In this project a biotechnological process has been developed in order to provide standardised, cost effective and reliable production of microalgae toxins. This includes the production of microalgae biomass, the harvesting of the microalgae and the chemical purification of the microalgae toxins. First charges of toxin standard material could be produced.

Funding:
This project was supported by the funding program „Company Innovation-Basic Innovation“ that is issued by the WTSH (Business Development and Technology Transfer Corporation).


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Title: Acceleration of hydrogenproduction in Cyanobacteria (HWT-Program, Innovationsstiftung Schleswig-Holstein).

Duration: 01.05.2005 – 31.12.2006.

Description:
The aim of this project was to increase the use of hydrogen as an alternative source of energy. Cyanobacteria, algae and plants are able to convert water and carbon dioxide with sunlight into chemical energy. In algae and cyanobacteria it is possible to produce hydrogen in certain steps of the process. It was evaluated if hydrogen can be produced from water and sunlight through photosynthesis in cyanobacteria. By genetical engeneering specific metabolic pathways can be redirected and are able to support accelerated hydrogen production.

Funding:
Als Kooperationspartner im Rahmen des Förderprogramms „Hochschule – Wirtschaft – Transfer“ (HWT). Antragssteller: Christian-Albrechts-Universität, AG Schulz, Herr Prof. Dr. Rüdiger Schulz.


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Title: An Innovation assistant was founded in our company through the Future program „Economics“ (WTSH).

Duration: 01.08.2008 bis 31.07.2010.

Description:
The aim of this project was the optimisation of production processes regarding energy efficiency and biological optimisation. The collection of data and the subsequent measurements enabled a successful reduction of production costs while maintaining an unchanged high level of product quality.

Funding:

in unserem Unternehmen wurde ein Innovationsassistent aus dem Zukunftsprogramm 'Wirtschaft' gefördert

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Title: Application of Pavlova microalgae as alternative feed in larval rearing.

Duration: 12.2010 – 12.2013

Description:
The main aim of the project is, that based on account of high growth rates in the marine aquaculture a rising need arises in suitable feeds for marine fish larvae. Up to now this need can be still provided by conventional dry mixing feeds. Marine microalgae are valid as ideal larva feeds above all in combination with natural live feed like rotifers and small saline crustaceans. Besides, the high need of the marine fish larvae must be covered by the feeds in both essential fatty acids Eicosapentaenoic acid and Docosahexaenoic acid. The few marine microalgae produced up to now for the feeding of marine fish larvae (above all Nannochloropsis and Isochrysis) contain in each case only one of both essential fatty acids. By contrast the microalgae Pavlova is able to synthesise both fatty acids in parallel. However, up to now the industrial production of this microalga is not possible particularly on account of its high sensitivity to shear forces and high temperatures (> 28°C). Therefore, in this project the process technology should be developed for the industrial production, harvest and product formulation for this algae. In addition extensive cultivation experiments are carried out by the project partner research and technology centre west coast of the university of Kiel in the lab (1 litre to 250 litres) with industrially relevant conditions from the greenhouse (among other things fluctuating light, temperature). In parallel BlueBioTech GmbH modifies different photobioreactor components for cultivation experiments with Pavlova-algae in the greenhouse. Moreover, suitable harvest and preservation procedures are developed by BlueBioTech for this algae. In the cultivation experiments in 250 l and 1000 l Pavlova algae are used in different product formulations by the cooperation partner society for marine aquaculture (GMA) for the enrichment of rotifers and small saline crustaceans and subsequent feeding of marine fish larvae. Finally the Pavlova-algae should be also used with the GMA in feeding experiments with marine fish to substitute for the fish oil in feeds for adult fish. Due to the restricted production of fish oil and a rising request of fish oil this is of essential importance for the backup of sufficient feed amounts for aquaculture. As a result of this project the company BlueBioTech would like to produce the Pavlova algae as a feed for marine fish larvae and in the longer term also as an alternative to fish oil for adult fish. Both would lead to a clear protection of natural resources and development of other potentials in marine aquaculture.

Funding:

This project is funded by the Deutsche Bundesstiftung Umwelt (DBU).

Project partners:

Forschungs- und Technologiezentrum Westküste der Christian-Albrechts-Universität zu Kiel
Hafentörn 1
25761 Büsum

GMA – Gesellschaft für Marine Aquakultur mbH
Hafentörn 3
25761 Büsum

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Title:
Development of Microalgae concentrates as feed for Copepods for application in marine aquaculture (MiCo).

Duration: 06.03.2012 – 05.03.2015.

Description:
Copepods are well known as optimal feed for larvae of many fishspecies. In general copepods can be produced based on marine microalgae. However the species of microalgae used plays an important role. The production of suitable microalgae is a great challenge for the hatcheries as most of these algae are difficult to produce. Suitable microalgae are not available on the market as a storable product. Hence the aim of this project is to evaluate the conditions for commercial production of algae suited for copepod production, to harvest these algae and formulate marketable products from it. First there will be growth experiments with different algae species. The biomass will be used to develop gentle harvesting techniques that do not destroy the algae. The formulation of the products will include fresh and frozen biomass. Based on these findings the algae will be grown in a greenhouse at 1000L capacity. The society for marine aquaculture (GMA) will test the algae as feed for copepods on a contract basis. In order to enable a quality control and optimization of the algae production the fatty acids of the algae and of the copepods will be determined analytically.

Funding:

This project is funded by the Bundesanstalt für Landwirtschaft und Ernährung.

Project partners:


GMA – Gesellschaft für Marine Aquakultur mbH
Hafentörn 3
25761 Büsum

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